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Experience

BIOCHEMIST March 2021 - May 2023

IBN Sina Diagnostic and Imaging Center, Dhanmondi, Dhaka

2020 - Present

ABEx Bio-Research Center. Dhaka, Bangladesh

Research Assistant 2019 - 2020

Bangladesh Council of Scientific and Industrial Research. Chittagong, BD

Education

Graduate student Aug 2023 - Now

Department of Genetics & Biochemistry, Clemson University, USA

B.Sc.(Hons.) Jan 2016 - Dec 2020

Department of Biochemistry and Biotechnology, USTC, BD

What I Can Do

Skills

Clinical Biochemsitry

Automated Biochemical and Immunological Assays

01
Molecular Biology

Conventional and RT PCR, Flow cytometry (FC)

02
Genomics

gene sequencing using ABI 3730 XL, Hitachi Ltd., USA

03
Computational Biology and Bioinformatics

Drug design, Protein networks, Homology modeling, docking, and simulation

04
Data analysis and Programing

Fundamental data analysis using Python and R programming.

05
Graphic Design

Digital painting, image preparation, and processing.

06
About Me

Md. Abul Kalam Azad

Program: Biochemistry and Biotechnology

Website: www.akazad.net

Phone: +8801836930655

Study: University of Science and Technology Chittagong

Degree: B.Sc.(Hons.)

Mail: makazad.ustc@gmail.com

Linkedin: @akazadbtech

Research gate: Md-Abul-Kalam-Azad-17

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Recent work completed

Researh Work showcase

Project 10.

Abstract Meat species identification and animal authentication in meat products is an important topic that should be addressed in order to promote fair trade and allow customers to make informed decisions. It is also a major concern for practicing religious people. Protein or DNA measurements are used in many analytical procedures. Electrophoretic, chromatographic, and immunological approaches based on protein fractions are typically ineffective in distinguishing closely related meat species in processed foods. Molecular techniques for meat species identification have gotten a lot of interest in recent years because of their high sensitivity and specificity, as well as their short processing time and inexpensive cost. This review discusses an overview of the most common DNA and PCR-based approaches used to validate the authenticity of meat and meat products to date.
Project 8.

AbstractFormulae display:MathJax Logo? Cancer is an umbrella term used to define various diseases with abnormal cell proliferation at the focal point. According to the WHO, cancer is the leading cause of death worldwide, with lung cancer being the second most common perpetrator after breast cancer. There are several proteins acting in harmony that lead to cancer. EGFR has been identified as one of the proteins that is linked to cell division, even when it is cancerous in nature. Cancer can be treated using therapeutic agents that target EGFR or their signaling networks. Available drugs that could inhibit EGFR have acquired resistance in most cases and multiple side effects on the human body. That is why phytochemicals are being studied for their role in this case. Around 8000 compounds were retrieved from our previously created phytochemdb database for their drug activity, and the 3D protein structure was collected from the protein data bank. The selected dataset of ligands was virtually screened through HTVS, SP, and XP to retain the top 4 hits. Molecular dynamics revealed the stability and flexibility of protein-(selected)ligand interactions. The non-bond interactions of each of the compounds with EGFR, such as Gossypetin interacting with active site MET769 and ASP831; Muxiangrine III interacting with MET769 and ASP831; Quercetagetin showing non-bonded interactions with GLU738, GLN767, and MET769 for >100% of the simulation timeframe These findings suggest further research into these compounds, which can yield a potential phytochemical drug against cancer.
Project .01

The frequent occurrence of heat and drought stress can severely reduce agricultural production of field crops. In comparison to a single stress, the combination of both heat (H) and drought (D) further reduce plant growth, survival and yield. This study aimed to explore the transcriptional responses of heat shock protein (HSP) and antioxidant genes under H combined D stress in perennial rye grass (PRG). The results demonstrated that oxidative stress indicators (hydrogen peroxide, lipid peroxidation) significantly increased, particularly in the case of combined H and D treatment, suggesting that oxidative stress-induced damage occurred in plants under the combined stresses
Project .04

The first major outbreak in Bangladesh was reported in 2008 in Rajshahi and Chapainawabgonj. It then re-emerged in 2013, 2014 and 2015 mostly in Dhaka and other parts of Bangladesh, with a notable outbreak in December 2016 according to a report. This is a statistical report on the data collected from chikungunya patients in some areas of Chittagong in relation to age, gender, location, weather and symptoms of the patients. The aim of the report is to assess the magnitude of the outbreak and an interest to identify the probable socio-environmental factors responsible for chikungunya in Chittagong.
Project .03

Abstract Fresh fruits and vegetables are an essential part of people's diet all over the world. Since they are typically eaten raw and often without heat treatment or thorough washing, so they serve as vectors for the transmission of pathogenic micro-organisms associated with human diseases. This study aimed at isolating and identifying the pathogenic bacteria from selected popular fresh fruits and vegetables which are known as street food. A total of 7 fresh fruits and vegetables were collected from different open markets of Chittagong under aseptic conditions. Selective and non-selective media were used to isolate and enumerate the bacteria. All the species were identified on the basis of morphology, biochemical tests (IMViC) and selective differential.
Project .02

ABSTRACT: Accurate identification of important plants is essential for their safety, efficacy, and herbal remedies. The study was aimed to identify 4 locally available medicinal plants using DNA barcoding approach. Genomic DNA was extracted from plant samples followed by their amplification by the conventional PCR approach. Short sequence diversity of standardized specific coding gene regions of matK gene of plastid genome was used to compare and differentiate the plant species. Subsequently, all the samples were purified and sequenced successfully. A phylogenetic tree was constructed to assess their cross-species relationship. All the samples showed a high similarity rate with their homologs after blasting them in the NCBI database. The phylogenetic study showed a distinguished relationship with each other. All the result indicates that the DNA barcoding approach could be successfully used for reliable identification of medicinal plants and matK gene is a good candidate for this approach.
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